Serial dilution: a practical study guide

Overview

A serial dilution is a sequence of dilution steps used to reduce sample concentration. At each step, a measured aliquot is transferred into a known volume of diluent. The overall dilution is the product of the individual step dilutions.

Key principles

For example, transferring 1 mL into 9 mL diluent gives a 1:10 dilution. Repeating this three times gives a 1:1,000 dilution. Mix each tube before the next transfer and use a fresh sterile tip. Follow your validated laboratory procedure for sample-specific handling.

Further reading

Use authoritative references alongside your course material and current validated laboratory procedures.

Read the original website article ↗

Continue exploring

Antibiotic resistance: causes, mechanisms & prevention →

Bioplastics from algae and bacteria →

Photosynthesis: steps, types & importance →